MYO1D Polyclonal antibody

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  WB analysis of mouse lung using 14028-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14028-1-AP (MYO1D antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14028-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14028-1-AP (MYO1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14028-1-AP

  IP result of anti-MYO1D (IP:14028-1-AP, 4ug; Detection:14028-1-AP 1:300) with mouse lung tissue lysate 4000ug.

MYO1D Polyclonal Antibody for WB, IP, ELISA

Cat No. 14028-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Synonyms

KIAA0727, MYO1D, myosin ID, myr4

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

14028-1-AP targets MYO1D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5176

  Product name: Recombinant human MYO1D protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-350 aa of BC030602

  Sequence: MAEQESLEFGKADFVLMDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKLLNIYGRDTIEQYKGRELYERPPHLFAIADAAYKAMKRRSKDTCIVISGESGAGKTEASKYIMQYIAAITNPSQRAEVERVKNMLLKSNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQPGERSFHSFYQLLQGGSEQMLRSLHLQKSLSSYNYIHVGAQLKSSINDAAEFRVVADAMKVIGFKPEEIQTVYKILAAILHLGNLKFVVDGDTPLIENGKVVSIIAELLSTKTDMVEKALLYRTVATGRDIIDKQHTEQEASYGRDAFAKAIYER

• Full Name: myosin ID
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030602
• Gene Symbol: MYO1D
• Gene ID (NCBI): 4642
• RRID: AB_2266818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94832
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
human	IHC	Cytoskeleton (Hoboken); Myosin VI is required for maintenance of brush border structure, composition, and membrane trafficking functions in the intestinal epithelial cell.; Authors - Hegan Peter S PS