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  • KD/KO Validated

MYO1E Polyclonal antibody

MYO1E Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17768-1-AP

Synonyms

HuncM IC, MYO1E, Myosin Ic, myosin IE



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17768-1-AP targets MYO1E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO1E fusion protein Ag12176
Full Name myosin IE
Calculated Molecular Weight 1108 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC098392
Gene Symbol MYO1E
Gene ID (NCBI) 4643
RRIDAB_2251045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO1E, also named as Myosin-Ie and MYO1C (distinct from the MYO1C gene located on chromosome 17), encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6.

Protocols

Product Specific Protocols
WB protocol for MYO1E antibody 17768-1-APDownload protocol
IHC protocol for MYO1E antibody 17768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration

Authors - Irving Ulises Martínez-Vargas
mouseWB

Eur J Immunol

The class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release and antigen presentation via MHC class II.

Authors - Jens Wenzel
  • KO Validated

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
humanWB,IF

Oncol Lett

Analysis and validation of the potential of the MYO1E gene in pancreatic adenocarcinoma based on a bioinformatics approach

Authors - Songbai Liu
  • KD Validated