MYO3A Polyclonal antibody

MYO3A Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28562-1-AP

Synonyms

DFNB30, MYO3A, myosin IIIA

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Validation Data Gallery

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  IHC staining of mouse eye using 28562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 28562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 28562-1-AP (MYO3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 28562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 28562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MYO3A antibody (28562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28562-1-AP targets MYO3A in IF, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO3A fusion protein Ag29335
• Full Name: myosin IIIA
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 190 kda
• GenBank Accession Number: NM_017433
• Gene Symbol: MYO3A
• Gene ID (NCBI): 53904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.