MYO5A Polyclonal antibody

MYO5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55318-1-AP

Synonyms

GS1, MYH12, MYO5A, Myosin 12, Myosin heavy chain 12, Myosin Va, Myoxin, MYR12

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Validation Data Gallery

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  WB analysis using 55318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55318-1-AP (MYO5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 55318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55318-1-AP targets MYO5A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin VA (heavy chain 12, myoxin)
• Calculated Molecular Weight: 215 kDa
• Observed Molecular Weight: 210-220 kDa
• GenBank Accession Number: NM_000259
• Gene Symbol: MYO5A
• Gene ID (NCBI): 4644
• RRID: AB_2918356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.