MYO5B Polyclonal antibody, PBS Only

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• MYO5B Polyclonal antibody, PBS Only (29996-1-PBS)

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  WB analysis using 29996-1-AP (same clone as 29996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29996-1-AP (MYO5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

MYO5B Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29996-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29996-1-PBS targets MYO5B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31590

  Product name: Recombinant human MYO5B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 940-1114 aa of NM_001080467

  Sequence: EFKTLSEQLSVTTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRKRVADLEQENALLKDEKEQLNNQILCQSKDEFAQNSVKENLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYP

• Full Name: myosin VB
• Calculated Molecular Weight: 214KD
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_001080467
• Gene Symbol: MYO5B
• Gene ID (NCBI): 4645
• RRID: AB_2935496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULV0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).