Tested Applications
| Positive WB detected in | mouse thymus tissue, HL-60 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3108 Product name: Recombinant human MYO9B protein Source: e coli.-derived, T-GST Tag: GST Domain: 1657-1980 aa of NM_004145.3 Sequence: DKALLCSVCKMTCHKKCVHKIQSHCSYTYGRKGEPGVEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPAAVKLENFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLEHLPEANHNSLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEISQLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEGVLNKSPKTRDIQEEELEVLLEEEAAGGDEDREKEILIERIQSIKEEKEDI Predict reactive species |
| Full Name | myosin IXB |
| Calculated Molecular Weight | 2023 aa, 223 kDa |
| Observed Molecular Weight | 230-250 kDa |
| GenBank Accession Number | NM_004145.3 |
| Gene Symbol | MYO9B |
| Gene ID (NCBI) | 4650 |
| RRID | AB_2148635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYO9B antibody 12432-1-AP | Download protocol |
| IP protocol for MYO9B antibody 12432-1-AP | Download protocol |
| WB protocol for MYO9B antibody 12432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYO9B Polyclonal antibody (Cat# 12432-1-AP) has 13 citations. Journals include Nature Communications, Journal of Clinical Investigation, Cell Reports, Journal of Bone and Mineral Research, Frontiers in Bioengineering and Biotechnology, Journal of Thrombosis and Haemostasis, Journal of Molecular Biology, European Journal of Neurology, Journal of Immunology, Journal of General Virology, and PLoS ONE. Research fields span cancer metastasis, bone biology, immunology, virology, neuropathy, and platelet biology.
| Species | Application | Title |
|---|---|---|
Nat Commun An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells
| ||
Nat Commun Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset | ||
J Clin Invest Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progression. | ||
Cell Rep MIRO2 promotes cancer invasion and metastasis via MYO9B suppression of RhoA activity | ||
Cell Rep Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.
| ||
J Bone Miner Res The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.
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Reviews
What customers say
The single review for this product (rated 3/5) was conducted on HUVEC cells using a 1:200 dilution for immunofluorescence and flow cytometry. The reviewer noted the presence of numerous speckles in the staining pattern, though the signal was also clearly observed at focal adhesions, consistent with published literature. Overall, the antibody performed adequately but showed some non-specific background that may require optimization.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (11-20-2023) | Lots of speckles, but also observed at focal adhesions where it is reported in the literature.
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