MYO9B Polyclonal antibody

• Featured Product
• KD/KO Validated

MYO9B Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 12432-1-AP

Synonyms

Unconventional myosin-IXb, Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein

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Validation Data Gallery

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  WB analysis of mouse thymus using 12432-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12432-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12432-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12432-1-AP (MYO9B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12432-1-AP

  IP result of anti-MYO9B (IP:12432-1-AP, 5ug; Detection:12432-1-AP 1:500) with HepG2 cells lysate 2800ug.

• 

  IHC staining of human tonsillitis using 12432-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12432-1-AP (MYO9B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HL-60 cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IF: See 5 publications below

Product Information

12432-1-AP targets MYO9B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO9B fusion protein Ag3108
• Full Name: myosin IXB
• Calculated Molecular Weight: 2023 aa, 223 kDa
• Observed Molecular Weight: 230-250 kDa
• GenBank Accession Number: BC018108
• Gene Symbol: MYO9B
• Gene ID (NCBI): 4650
• RRID: AB_2148635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset; Authors - Jing Zhang
human	WB	J Thromb Haemost; The RhoA regulators Myo9b and GEF-H1 are targets of cyclic nucleotide-dependent kinases in platelets.; Authors - Shane Comer
human	WB,IF	Cell Rep; Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells.; Authors - Katarzyna A Makowska ; KD Validated
mouse	IF	J Bone Miner Res; The RhoGAP Myo9b Promotes Bone Growth by Mediating Osteoblastic Responsiveness to IGF-1.; Authors - Brooke K McMichael ; KO Validated
mouse	WB	J Immunol; TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.; Authors - Feng-Shuang Yi
human	WB	J Mol Biol; Non-canonical Myo9b-RhoGAP accelerates RhoA GTP hydrolysis by a dual-arginine-finger mechanism.; Authors - Fengshuang Yi ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Lots of speckles, but also observed at focal adhesions where it is reported in the literature.

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1 in 200
• Cell Tissue Type: HUVEC

MYO9B Antibody Immunofluorescence,Flow Cytometry validation (1 in 200 dilution) in HUVEC (Cat no:12432-1-AP)