|Positive WB detected in||mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue|
|Positive IHC detected in||mouse embryo tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18943-1-AP targets MYOD1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MYOD1 fusion protein Ag13512|
|Full Name||myogenic differentiation 1|
|Calculated molecular weight||320 aa, 35 kDa|
|Observed molecular weight||35-45 kDa|
|GenBank accession number||BC064493|
|Gene ID (NCBI)||4654|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)
Cell Death Dis
MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation.
IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway.
Exp Cell Res
Guanine-rich RNA binding protein GRSF1 inhibits myoblast differentiation through repressing mitochondrial ROS production.
J Agric Food Chem
Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.
Growth factors improve the proliferation of Jeju black pig muscle cells by regulating MyoD and growth-related genes.
Aging (Albany NY)
Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cassandra (Verified Customer) (02-04-2021)
This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.