MYOD1 Polyclonal antibody

MYOD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 18943-1-AP

Synonyms

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18943-1-AP targets MYOD1 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYOD1 fusion protein Ag13512
Full Name myogenic differentiation 1
Calculated molecular weight 320 aa, 35 kDa
Observed molecular weight 35-45 kDa
GenBank accession numberBC064493
Gene symbol MYOD1
Gene ID (NCBI) 4654
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Protocols

Product Specific Protocols
WB protocol for MYOD1 antibody 18943-1-APDownload protocol
IHC protocol for MYOD1 antibody 18943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Cell Death Dis

MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation.

Authors - Meng-Jie Zhao
humanWB

Skelet Muscle

IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway.

Authors - Dong Liu
mouseWB

Exp Cell Res

Guanine-rich RNA binding protein GRSF1 inhibits myoblast differentiation through repressing mitochondrial ROS production.

Authors - Wenxin Yin
mouseWB

J Agric Food Chem

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Authors - Luchu Zhang
pigWB, IF

Anim Biosci

Growth factors improve the proliferation of Jeju black pig muscle cells by regulating MyoD and growth-related genes.

Authors - Jinryong Park
humanWB

Aging (Albany NY)

Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging.

Authors - Yan Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cassandra (Verified Customer) (02-04-2021)

This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Satellite cell
MYOD1 Antibody Immunofluorescence validation (1:100 dilution) in Satellite cell (Cat no:18943-1-AP)