MYOF Polyclonal antibody, PBS Only

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• MYOF Polyclonal antibody, PBS Only (19548-1-PBS)

• 

  WB analysis using 19548-1-AP (same clone as 19548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (MYOF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19548-1-AP (same clone as 19548-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19548-1-AP (same clone as 19548-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Myoferlin antibody (19548-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

MYOF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19548-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19548-1-PBS targets MYOF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myoferlin
• Calculated Molecular Weight: 235 kDa
• Observed Molecular Weight: 235-250 kDa
• GenBank Accession Number: NM_133337
• Gene Symbol: MYOF
• Gene ID (NCBI): 26509
• RRID: AB_10642558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZM1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.