MYOF Recombinant antibody, PBS Only

• 

  WB analysis using 82917-2-RR (same clone as 82917-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82917-2-RR (Myoferlin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82917-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82917-2-RR (same clone as 82917-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYOF antibody (82917-2-RR, Clone: 230213E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82917-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82917-2-RR (same clone as 82917-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYOF Recombinant antibody (82917-2-RR, Clone:230213E10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82917-2-PBS in a different storage buffer formulation.

MYOF Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82917-2-PBS

Clone No.230213E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Myoferlin, KIAA1207, Fer-1-like protein 3, FER1L3, Fer 1 like protein 3

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Product Information

82917-2-PBS targets MYOF in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myoferlin
• Calculated Molecular Weight: 235 kDa
• Observed Molecular Weight: 230-240 kDa
• GenBank Accession Number: NM_133337
• Gene Symbol: MYOF
• Gene ID (NCBI): 26509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZM1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR.