MYOM1 Polyclonal antibody

MYOM1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22084-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse heart using 22084-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat heart using 22084-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 22084-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 22084-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 22084-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 22084-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, rat heart tissue
Positive IHC detected in	mouse heart tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22084-1-AP targets MYOM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17263 Product name: Recombinant human MYOM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1230-1589 aa of BC116183 Sequence: TNHSTVVLVGDVFKKLQKEAEFQRQEWIRKQGPHFVEYLSWEVTGECNVLLKCKVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGIYEVILKDDRGKDKSRLKLVDEAFKELMMEVCKKIALSATDLKIQSTAEGIQLYSFVTYYVEDLKVNWSHNGSAIRYSDRVKTGVTGEQIWLQINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAYDEAYAEFQRLKQAAIAEKNRARVLGGLPDVVTIQEGKALNLTCNVWGDPPPEVSWLKNEKALASDDHCNLKFEAGRTAYFTINGVSTADSGKYGLVVKNKYGSETSDFTVSVFIPEEEARMAALESLKGGKKAK Predict reactive species
Full Name	myomesin 1, 185kDa
Calculated Molecular Weight	1685 aa, 188 kDa
Observed Molecular Weight	170-180 kDa
GenBank Accession Number	BC116183
Gene Symbol	MYOM1
Gene ID (NCBI)	8736
RRID	AB_2878989
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P52179
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent.

Protocols

Product Specific Protocols
IHC protocol for MYOM1 antibody 22084-1-AP	Download protocol
WB protocol for MYOM1 antibody 22084-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The MYOM1 Polyclonal antibody (Cat# 22084-1-AP) has 1 total citation. It was cited in BMC Microbiology (Impact Factor 5.4), published in November 2025. The associated research focuses on reproductive tract microbiota dysbiosis in ovarian endometrioma and adenomyosis, specifically examining multi-site 16S rRNA profiling and the functional impact of key bacterial species on human endometrial stromal cells. The antibody was used in an ELISA application with human samples.

Species	Application	Title
human	ELISA	BMC Microbiol Reproductive tract microbiota dysbiosis in ovarian endometrioma and adenomyosis: multi-site 16S rRNA profiling and functional impact of key bacterial species on human endometrial stromal cells. Authors - Junchi Li View Article