Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10731-1-AP targets MYOT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1112 Product name: Recombinant human MYOT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 191-498 aa of BC005376 Sequence: GPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQDAIQEKFYPPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL Predict reactive species |
| Full Name | myotilin |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 55-57 kDa, 35 kDa |
| GenBank Accession Number | BC005376 |
| Gene Symbol | MYOT |
| Gene ID (NCBI) | 9499 |
| RRID | AB_2297956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYOT antibody 10731-1-AP | Download protocol |
| WB protocol for MYOT antibody 10731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Myotilin antibody (Cat# 10731-1-AP) has 12 total citations published across 11 journals, including Neurology, Annals of Neurology, Journal of Cachexia, Sarcopenia and Muscle, European Journal of Neurology, American Journal of Pathology, Human Molecular Genetics, and eLife. Associated research fields include neuromuscular disorders, hereditary myopathies, muscular dystrophy, protein aggregation diseases, and heart failure with preserved ejection fraction.
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction-Regulating Proteins in a Rat Model of HFpEF | ||
Neurology Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. | ||
Neurol Neuroimmunol Neuroinflamm Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy: Practical Importance of Anti-α-Actinin Immunostaining | ||
Int J Mol Sci Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle | ||
