MYOZ3 Polyclonal antibody

MYOZ3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 23880-1-AP

Synonyms

Calsarcin 3, CS 3, CS3, FATZ related protein 3, FRP3, MYOZ3, myozenin 3

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Validation Data Gallery

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  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23880-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23880-1-AP is MYOZ3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYOZ3 fusion protein Ag20839
• Full Name: myozenin 3
• Calculated Molecular Weight: 251 aa, 27 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC074870
• Gene Symbol: MYOZ3
• Gene ID (NCBI): 91977
• RRID: AB_2879347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Martina (Verified Customer) (07-03-2023)

IF protocol for fixed cryosection of mouse skeletal muscle: after a quick permeabilization step with Triton 0,3% in PBS, block with BSA 0,5% and GS 10% in PBS for at least 2 hours at room temperature. Then incubate with primary antibody 1:100 in blocking solution overnight at +4°C. The day after, incubate with secondary antibody 1:200 in blocking solution for 2 hours at room temperature. WB protocol for mouse skeletal muscle proteins: block the membrane with 5% milk in TBS-T for at least 2 hours at room temperature; then incubate with primary antibody 1:1000 in 5% milk in TBS-T at +4°C overnight. The day after, incubate with secondary antibody 1:2000 in 5% milk in TBS-T for 2 hours at room temperature. Developing the membrane, you will see double bands between 39 and 28 kDa.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: Murine skeletal muscle

MYOZ3 Antibody Western Blot,Immunofluorescence validation ( dilution) in Murine skeletal muscle (Cat no:23880-1-AP)