MYPN-Specific Polyclonal antibody

MYPN-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16180-1-AP

Synonyms

145 kDa sarcomeric protein, MYOP, myopalladin, MYPN

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Validation Data Gallery

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  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16180-1-AP

  Immunofluorescent analysis of HepG2 cells using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16180-1-AP targets MYPN-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myopalladin
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_032578
• Gene Symbol: MYPN
• Gene ID (NCBI): 84665
• RRID: AB_2146767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

Publications

Species	Application	Title
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen