MYPT1 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66506-1-Ig |CloneNo.: 2A1A9

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Freight/Packing

Con: 150 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
Human

Positive WB detected in:
Raji cells,HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution:
WB : 1:2000-1:12000

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG3

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
protein phosphatase 1, regulatory (inhibitor) subunit 12A

Calculated molecular weight:
1030aa,115 kDa

Observed molecular weight:
115 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PPP1R12A

Synonyms
MBS, MYPT1, PPP1R12A
Background

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.


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