MYPT1 Monoclonal antibody

MYPT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A1A9

Cat no : 66506-1-Ig

Synonyms

MBS, MYPT1, PPP1R12A



Tested Applications

Positive WB detected inRaji cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Sample-dependent, check data in validation data gallery

Product Information

66506-1-Ig targets MYPT1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen MYPT1 fusion protein Ag17496
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Calculated molecular weight 1030 aa, 115 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC111752
Gene symbol PPP1R12A
Gene ID (NCBI) 4659
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

Protocols

Product Specific Protocols
WB protocol for MYPT1 antibody 66506-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols