WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.
WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.
WB analysis of HeLa using 20078-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20078-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 20078-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20078-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20078-1-AP
IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A431 using 20078-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
A549 cells, HepG2 cells, HeLa cells, HEK-293 cells
Positive IP detected in
HeLa cells
Positive IHC detected in
mouse kidney tissue, human spleen tissue, mouse small intestine tissue, human pancreas cancer tissue, human urothelial carcinoma tissue, rat kidney tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.
Protocols
Product Specific Protocols
WB protocol for MYSM1-Specific antibody 20078-1-AP
WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.
WB analysis of HeLa using 20078-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20078-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 20078-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20078-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse kidney using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 20078-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HeLa using 20078-1-AP
IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.
IP experiment of HeLa using 20078-1-AP
IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:1000) with HeLa cells lysate 1200 ug.
IF/ICC Figures
IF Staining of A431 using 20078-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using MYSM1-Specific antibody (20078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
MYSM1-Specific Polyclonal antibody
Catalog Number
20078-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet