MYSM1-Specific Recombinant monoclonal antibody

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  WB analysis using 85927-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 85927-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85927-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85927-1-RR (MYSM1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MYSM1-Specific Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85927-1-RR

Clone No.243158A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MYSM1, 2A DUB, 2ADUB, 2A-DUB, Deubiquitinase MYSM1

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, A549 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85927-1-RR targets MYSM1-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Myb-like, SWIRM and MPN domains 1
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: NM_001085487
• Gene Symbol: MYSM1
• Gene ID (NCBI): 114803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5VVJ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.