Tested Applications
| Positive WB detected in | HeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human thyroid cancer tissue, mouse stomach tissue, rat stomach tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13751-1-AP targets MYST2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4693 Product name: Recombinant human MYST2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC032640 Sequence: MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHDESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKHERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRHQAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEF Predict reactive species |
| Full Name | MYST histone acetyltransferase 2 |
| Calculated Molecular Weight | 611 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC032640 |
| Gene Symbol | MYST2 |
| Gene ID (NCBI) | 11143 |
| RRID | AB_2266703 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95251 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYST2 antibody 13751-1-AP | Download protocol |
| IHC protocol for MYST2 antibody 13751-1-AP | Download protocol |
| IP protocol for MYST2 antibody 13751-1-AP | Download protocol |
| WB protocol for MYST2 antibody 13751-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYST2 Polyclonal antibody (Cat# 13751-1-AP) has 28 citations published in journals including Cancer Cell, Cell Research, Cancer Discovery, Autophagy, Nucleic Acids Research, Cell Reports, eLife, FASEB Journal, and Journal of Biological Chemistry, among others. Research fields span epigenetics and histone acetylation, cancer biology (lung, breast, liver, leukemia), RNA modification, ferroptosis, stem cell biology, cardiovascular disease, and proteomics. Applications cited include WB, IP, IHC, IF, ChIP, and CoIP.
| Species | Application | Title |
|---|---|---|
Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.
| ||
Cell Res Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner | ||
Cancer Discov Functional mapping of epigenomic regulators uncovers coordinated tumor suppression by the HBO1 and MLL1 complexes | ||
Cancer Commun (Lond) Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma | ||
Cancer Res Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells. | ||
Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. |
Reviews
What customers say
The single review reports excellent performance for both immunoprecipitation and Western blot. The antibody effectively pulled down KAT7 along with other complex members in IP, and delivered a strong signal in Western blot at a 1:2000 dilution. The reviewer rated it 5 stars.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jessica (Verified Customer) (10-06-2025) | Effectively pulled down KAT7 in IP, as well as other complex members. Also worked well in western blot at 1:2000 dilution with high signal
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