MYT1 Polyclonal antibody

MYT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26204-1-AP

Synonyms

MYTI, Myelin transcription factor I, MTF1, KIAA1050, KIAA0835

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 26204-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26204-1-AP (MYT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26204-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26204-1-AP targets MYT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYT1 fusion protein Ag24580
• Full Name: myelin transcription factor 1
• Calculated Molecular Weight: 591aa,65 kDa; 1121aa,122 kDa
• Observed Molecular Weight: 122 kDa
• GenBank Accession Number: BC062313
• Gene Symbol: MYT1
• Gene ID (NCBI): 4661
• RRID: AB_2880424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Evid Based Complement Alternat Med; The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma; Authors - Chun-Peng Zhang
human	IF	Adv Sci (Weinh); Integration of Functional Human Auditory Neural Circuits Based on a 3D Carbon Nanotube System; Authors - Yiyun Lou