MYT1L Polyclonal antibody

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• MYT1L Polyclonal antibody (25234-1-AP)

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• KD/KO Validated

MYT1L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25234-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NZF1, MyT1-L, MyT1 L, Myelin transcription factor 1-like protein, KIAA1106

• 

  WB analysis of HepG2 using 25234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25234-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25234-1-AP targets MYT1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18698

  Product name: Recombinant human MYT1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 160-473 aa of BC137273

  Sequence: EEEEEEEENEDHQMNCHNTRIMQDTEKDDNNNDEYDNYDELVAKSLLNLGKIAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSENMNDRNYADSMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQERNPQQNMNIRQHVRPEEDFPGRTPDRNYSDMLNLMRLEEQLSPRSRVFASCAKEDGCHERDDDTTSVNSDRSEEVFDMTKGNLTLLEKAIALETERAKAMREKMAMEAGRRDNMRSYEDQSPRQL

• Full Name: myelin transcription factor 1-like
• Calculated Molecular Weight: 1186 aa, 133 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC137273
• Gene Symbol: MYT1L
• Gene ID (NCBI): 23040
• RRID: AB_2879978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

MYT1L Polyclonal antibody (Cat# 25234-1-AP) has 7 citations published in Nature Neuroscience, Neuron, Cell Proliferation, Genome Research, Brain Research Bulletin, Journal of Biological Chemistry, and bioRxiv. The research fields span neurodegeneration (Huntington's disease), brain development and neuronal maturation, glioma biology, and alternative splicing during neurodevelopment.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Neurosci; Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy; Authors - Young Mi Oh
mouse	WB,IF	Neuron; A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation.; Authors - Jiayang Chen
mouse	IF	Cell Prolif; Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors; Authors - Yuhang Huang
mouse	IP	Genome Res; MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain; Authors - Jiayang Chen
human	WB	Brain Res Bull; miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L; Authors - Zhengtao Yu ; KD Validated
mouse	WB	J Biol Chem; Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis; Authors - Masayoshi Nagai