MYT1L Polyclonal antibody

• 

  WB analysis of HepG2 using 25234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25234-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25234-1-AP (MYT1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25234-1-AP (MYT1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

MYT1L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25234-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NZF1, MyT1-L, MyT1 L, Myelin transcription factor 1-like protein, KIAA1106

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

25234-1-AP targets MYT1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18698

  Product name: Recombinant human MYT1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 160-473 aa of BC137273

  Sequence: EEEEEEEENEDHQMNCHNTRIMQDTEKDDNNNDEYDNYDELVAKSLLNLGKIAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSENMNDRNYADSMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQERNPQQNMNIRQHVRPEEDFPGRTPDRNYSDMLNLMRLEEQLSPRSRVFASCAKEDGCHERDDDTTSVNSDRSEEVFDMTKGNLTLLEKAIALETERAKAMREKMAMEAGRRDNMRSYEDQSPRQL

• Full Name: myelin transcription factor 1-like
• Calculated Molecular Weight: 1186 aa, 133 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC137273
• Gene Symbol: MYT1L
• Gene ID (NCBI): 23040
• RRID: AB_2879978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Neuron; A MYT1L syndrome mouse model recapitulates patient phenotypes and reveals altered brain development due to disrupted neuronal maturation.; Authors - Jiayang Chen
mouse	IF	Cell Prolif; Direct reprogramming of fibroblasts into spiral ganglion neurons by defined transcription factors; Authors - Yuhang Huang
human	WB	Brain Res Bull; miRNA-338-3p inhibits glioma cell proliferation and progression by targeting MYT1L; Authors - Zhengtao Yu ; KD Validated
mouse	WB	J Biol Chem; Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis; Authors - Masayoshi Nagai
human	IF	Nat Neurosci; Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy; Authors - Young Mi Oh
mouse	IP	Genome Res; MYT1L is required for suppressing earlier neuronal development programs in the adult mouse brain; Authors - Jiayang Chen