Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10004-2-Ig targets Marcks in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | myristoylated alanine rich protein kinase C substrate |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC046601 |
| Gene Symbol | Marcks |
| Gene ID (NCBI) | 17118 |
| RRID | AB_2140311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26645 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Marcks antibody 10004-2-Ig | Download protocol |
| WB protocol for Marcks antibody 10004-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MARCKS antibody (Cat# 10004-2-Ig) has 19 citations across journals including PNAS, Dev Cell, Cell Reports, Current Biology, Journal of Biological Chemistry, Molecular Biology of the Cell, and Journal of Virology. Research fields span oncology (hepatocellular, cervical, gastric, and melanoma cancers), neuroscience, cell biology, virology, endocrinology, and developmental biology.
| Species | Application | Title |
|---|---|---|
NPJ Precis Oncol Integrated multi-omics analysis reveals that MARCKS reprograms the immunosuppressive microenvironment to drive hepatocellular carcinoma progression.
| ||
Proc Natl Acad Sci U S A MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability. | ||
Dev Cell An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration | ||
Clin Transl Med Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer | ||
Curr Biol Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons | ||
Cell Rep Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon. |
Reviews
What customers say
The single review for this product is from Sana Fattah (October 2025), who rated it 5 stars. She used it at a 1:100 dilution for immunohistochemistry with lot 00001460 and commented simply: "Worked nicely!" Overall, the feedback is positive, indicating satisfactory performance in IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sana (Verified Customer) (10-31-2025) | Worked nicely!
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