|Positive WB detected in||mouse brain tissue|
|Positive IHC detected in||mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10004-2-Ig targets Marcks in WB, IP, IHC, IF,ELISA applications and shows reactivity with mouse samples.
|Cited Reactivity||bovine, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||myristoylated alanine rich protein kinase C substrate|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC046601|
|Gene ID (NCBI)||17118|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS
J Biol Chem
The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.
Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains.
Mol Biol Cell
Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding.
Accelerated proliferation and differential global gene expression in pancreatic islets of five-week-old heterozygous Men1 mice: Men1 is a haploinsufficient suppressor.
GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.
J Cell Sci
Dynamic adhesions and MARCKS in melanoma cells.