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  • KD/KO Validated

Marcks Polyclonal antibody

Marcks Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10004-2-Ig

Synonyms

Macs, Marcks, PKCSL



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10004-2-Ig targets Marcks in WB, IP, IHC, IF,ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivity bovine, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name myristoylated alanine rich protein kinase C substrate
Calculated molecular weight 39 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC046601
Gene symbol Marcks
Gene ID (NCBI) 17118
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

Protocols

Product Specific Protocols
WB protocol for Marcks antibody 10004-2-IgDownload protocol
IHC protocol for Marcks antibody 10004-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Biol Chem

The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.

Authors - Zhenzhen Chen
  • KO Validated
ratWB,IF

Neurosci Lett

Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains.

Authors - Musse Abdiwahab A AA
humanWB,IF

Mol Biol Cell

Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding.

Authors - Gatlin Jesse C JC
mouseWB,IHC

Endocrinology

Accelerated proliferation and differential global gene expression in pancreatic islets of five-week-old heterozygous Men1 mice: Men1 is a haploinsufficient suppressor.

Authors - Lejonklou Margareta H MH
humanWB,IP

Carcinogenesis

GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.

Authors - Bai Tao T
human,mouseIF

J Cell Sci

Dynamic adhesions and MARCKS in melanoma cells.

Authors - Estrada-Bernal Adriana A