Mayer's Hematoxylin Solution

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• Mayer's Hematoxylin Solution (PR30004)

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  FFPE human placenta tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE human ovary tumor tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE human appendicitis tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE MCF-7 cell line was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE mouse small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE rat small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE rat tongue tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  FFPE rat brain tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  Frozen mouse liver tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

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  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody). PR30004 (Mayer's Hematoxylin) was used for counter staining

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  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) . PR30004 (Mayer's Hematoxylin) was used for counter staining.

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Cat No. PR30004

Publications

18

Subject

Tissue and cells

Specificity

Non-toxic chemicals, simple, time-saving

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Product Information

Proteintech's PR30004 (Mayer's Hematoxylin Solution) can be used for tissue and cell staining. Mayer's Hematoxylin can stain the cell nucleus to produce a blue color. The formula of this product has been optimized to avoid using toxic chemicals, e.g., mercury or methanol. Differentiation and bluing are not needed. The staining is simple and time-saving. 

The product is provided in a ready-to-use format. No further dilution is required. Each milliliter of this product can stain ~10 slides.

Component	Size
Mayer's Hematoxylin Solution	10 mL  (Dropper bottle)	125 mL (Narrow-neck bottle)	500 mL (Narrow-neck bottle)

Storage

Ambient. Stable for one year.

Usage

IHC counter staining:  

After immunohistochemistry signal development, apply the product to the tissue slides to make sure the tissues are fully covered. After staining for 1-2 minutes, rinse the slides thoroughly with TBS bluing buffer for 1-2 minutes, followed by immersing in distilled water for 2 minutes, or rinse the slides in distilled water, followed by immersing in a saturated Na₂HPO₄ solution for 2 minutes. Once the slides turn blue, they can be washed, dehydrated, cleared, mounted, and observed.

H&E staining: 

1. For FFPE samples: Immerse slides in xylene for 10 minutes. Repeat in fresh xylene again for another 10 minutes. Then rehydrate the slides by sequentially incubating in 100%, 95%, 80%, and 60% ethanol solutions for 5 minutes each. Finally, wash the slides with distilled water for 2 minutes.

2. Apply the product onto the tissue slides and make sure the tissues are fully covered. After staining for 1-2 minutes, rinse the slides thoroughly with TBST bluing buffer for 1-2 minutes followed by immersing in distilled water for 2 minutes, or rinse the slides in distilled water followed by immersing in a saturated Na₂HPO₄ solution for 2 minutes. Apply Eosin staining then. 

3. For frozen samples: Wash the samples with distilled water. Then, perform staining using the same protocol as above. 

4. For cell samples: Fix the cells with 4% PFA for 10 minutes or longer. After fixation, wash the cells twice with distilled water for 2 minutes. Then, stain with the same protocol as above. If the cells are embedded with paraffin, please de-paraffin using the FFPE sample protocol above.

Cautions

1. Stain with 1-2 samples to make sure the staining achieves optimal results. 

2. Wear a lab coat and gloves during operation.

Cited in Article as

PR30004, Mayer's Hematoxylin Solution, Proteintech, IL, USA

Publications

Application	Title
	Int J Nanomedicine; Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study; Authors - Pengcheng Zhang
	Free Radic Biol Med; PINK1/PARK2 dependent mitophagy effectively suppresses NLRP3 inflammasome to alleviate acute pancreatitis.; Authors - Jie Zhang
IHC	Front Med (Lausanne); Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome via Activation of Autophagy.; Authors - Chunyan Li
	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
	Dis Markers; Targeting Circulating lncRNA ENST00000538705.1 Relieves Acute Coronary Syndrome via Modulating ALOX15.; Authors - Hao Chen
	Evid Based Complement Alternat Med; Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress.; Authors - Hui Peng