MCP-1/CCL2 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• MCP-1/CCL2 Recombinant monoclonal antibody (83157-5-RR)

MCP-1/CCL2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83157-5-RR Copied!

Clone No. 230483F11

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

• 

  WB analysis of RAW 264.7 using 83157-5-RR

  Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83157-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for MCP-1/CCL2 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83157-5-RR targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: chemokine (C-C motif) ligand 2
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM-011333
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 20296
• RRID: AB_3670851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10148
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publications

What published studies show

The MCP-1/CCL2 Recombinant monoclonal antibody (230483F11), catalog number 83157-5-RR, has a total of 3 citations. These appear in Research, British Journal of Pharmacology, and Environmental Toxicology. The associated research fields include renal tubule injury and calcium oxalate crystal formation, cardioprotection after myocardial infarction, and endothelial cell inflammation induced by indoxyl sulfate.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Research (Wash D C); Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation; Authors - Xi Jin
		Br J Pharmacol; Tyrosine-protein phosphatase non-receptor type 2 deficiency in macrophages diminishes the anti-inflammatory and cardioprotective effects of gabapentin after myocardial infarction.; Authors - Zhenjun Li
human	WB	Environ Toxicol; Luteolin Inhibits Indoxyl Sulfate-Induced ICAM-1 and MCP-1 Expression by Inducing HO-1 Expression in EA.hy926 Human Endothelial Cells; Authors - Li-Chien Chang