Mmp9 Recombinant monoclonal antibody

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  WB analysis using 83768-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83768-3-RR (Mmp9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83768-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83768-3-RR against Rat Mmp9 were performed. The affinity constant is below 1 pM.

Mmp9 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83768-3-RR

Clone No.240895G9

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

matrix metallopeptidase 9, 240895G9

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

83768-3-RR targets Mmp9 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: matrix metallopeptidase 9
• Calculated Molecular Weight: 79kd
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: NM_031055.2
• Gene Symbol: Mmp9
• Gene ID (NCBI): 81687
• RRID: AB_3671362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50282
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mmp9 (Matrix metalloproteinase-9) belongs to matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. The immunogen sequence of this antibody is of rat origin.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Transl Oncol; Tumor-stroma proportion is associated with increased M2 macrophage abundance and predicts the resistance to immune checkpoint blockade in breast cancer; Authors - Yincheng Liu
rat	WB	Arthritis Res Ther; RTA 408 attenuates TBHP-Induced apoptosis in nucleus pulposus cells via Nrf2/ARE and NF-κB signaling pathways: in vitro and in vivo evidence for mitigating rats' intervertebral disc degeneration.; Authors - Weibin Chen