Mono-Methyl-Histone H3 (Lys36) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 82825-2-RR (same clone as 82825-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82825-2-RR (same clone as 82825-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys36) antibody (82825-2-RR, Clone: 241138B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82825-2-RR (same clone as 82825-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of peptide using 82825-2-RR (same clone as 82825-2-PBS)

  Dot blot analysis was used to confirm the specificity of Mono-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 82825-2-RR (same clone as 82825-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys36) (82825-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82825-2-PBS in a different storage buffer formulation.

Mono-Methyl-Histone H3 (Lys36) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 82825-2-PBS

Clone No.241138B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

H3 Me Lys36, H3 MeK36, H3 Methyl K36, H3 Methyl Lys36, H3 Methyl Lysine36

---

Product Information

82825-2-PBS targets Mono-Methyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.