Mono-Methyl-Histone H3 (Lys4) Recombinant monoclonal antibody

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  WB analysis using 84740-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84740-1-RR (Mono-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84740-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Mono-Methyl-Histone H3 (Lys4) antibody (84740-1-RR, Clone: 241940G8 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 84740-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Mono-Methyl-Histone H3 (Lys4) antibody (84740-1-RR, Clone: 241940G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of peptide using 84740-1-RR

  Dot blot analysis was used to confirm the specificity of 84740-1-RR Mono-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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  ChIP experiment of HeLa using 84740-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys4) (84740-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Mono-Methyl-Histone H3 (Lys4) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Cat No. 84740-1-RR

Clone No.241940G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

H3, H3 Me Lys4, H3 MeK4, H3 Methyl K4, H3 Methyl Lys4

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells, U-87 MG cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells
• Positive Dot Blot detected in: peptide
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1250-1:5000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84740-1-RR targets Mono-Methyl-Histone H3 (Lys4) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3672125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.