Mouse ACBP/DBI ELISA Kit
Cat no : KE10190
Synonyms
ACBP, Acyl-CoA-binding protein, DBI, Diazepam-binding inhibitor, Endozepine
Validation Data Gallery
Product Information
KE10190 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse ACBP/DBI ELISA kit is to be used to detect and quantify protein levels of endogenous mouse ACBP/DBI. The assay recognizes mouse ACBP/DBI. An antibody specific for mouse ACBP/DBI has been pre-coated onto the microwells. The mouse ACBP/DBI protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse ACBP/DBI is added to detect the captured mouse ACBP/DBI protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse ACBP/DBI ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13167 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 95% | 93%-96% |
| Mouse serum | 93% | 86%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 11.52 | 0.95 | 8.26 |
| 2 | 8 | 2.52 | 0.13 | 5.26 |
| 3 | 8 | 1.30 | 0.08 | 6.19 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.00 | 1.10 | 9.97 |
| 2 | 16 | 2.41 | 0.16 | 6.84 |
| 3 | 16 | 1.27 | 0.09 | 6.73 |
Background Information
ACBP/DBI (acyl-CoA binding protein/Diazepam-binding inhibitor) is a widely expressed protein that binds long-chain fatty acyl-CoA esters and plays a role in fatty acyl-CoA transport and pool formation. ACBP/DBI is critical to cellular proliferation, differentiation, mitochondrial functions, and autophagy. Deletion of ACBP in mouse results in sebocyte hyperplasia and sparse, matted hair with a greasy appearance.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACBP, Acyl-CoA-binding protein, DBI, Diazepam-binding inhibitor, Endozepine |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.