Mouse Angiopoietin-like 3/ANGPTL3 ELISA Kit
Cat no : KE10110
Synonyms
angiopoietin like 3, Angptl3, hypl
Validation Data Gallery
Product Information
KE10110 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Angiopoietin-like 3/ANGPTL3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Angiopoietin-like 3/ANGPTL3. The assay recognizes mouse Angiopoietin-like 3/ANGPTL3. An antibody specific for mouse Angiopoietin-like 3/ANGPTL3 has been pre-coated onto the microwells. The mouse Angiopoietin-like 3/ANGPTL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Angiopoietin-like 3/ANGPTL3 is added to detect the captured mouse Angiopoietin-like 3/ANGPTL3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Angiopoietin-like 3/ANGPTL3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 8.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30924 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 86%-107% |
| Cell culture supernatants | 93% | 83%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,136.9 | 43.9 | 3.9 |
| 2 | 20 | 249.8 | 4.3 | 1.7 |
| 3 | 20 | 63.7 | 2.0 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 983.4 | 89.1 | 9.1 |
| 2 | 24 | 239.4 | 11.0 | 4.6 |
| 3 | 24 | 62.0 | 1.9 | 3.0 |
Background Information
ANGPTL3 is a secreted glycoprotein primarily synthesized by hepatocytes and belongs to the angiopoietin-like protein family. ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis, and hematopoietic function, and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. ANGPTL3 inhibits low-density lipoprotein (LDL) and endothelial lipase (EL), two enzymes that hydrolyze triglycerides and phospholipids in plasma lipoproteins. ANGPTL3-deficient mice showed low plasma HDL cholesterol and HDL phospholipid (PL). ANGPTL3 can also regulate triglyceride metabolism by inhibiting lipoprotein lipase (LPL). ANGPTL3-deficient mice exhibit hypotriglyceridemia and elevated LPL activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | angiopoietin like 3, Angptl3, hypl |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomaterials Dual-functional poly(disulfide) enables "Once-and-for-all" atherosclerosis therapy via precision hepatocyte base editing. |