Speedy™ Mouse Angiopoietin-like 3/ANGPTL3 One-Step ELISA Kit
Cat no : SE60016
Synonyms
Angptl3, angiopoietin like 3
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Angiopoietin-like 3/ANGPTL3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 4.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30924 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 89% | 87%-92% |
| Cell culture supernatant | 86% | 77%-91% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 429.5 | 10.8 | 2.5 |
| 2 | 8 | 115.0 | 8.5 | 7.4 |
| 3 | 8 | 61.8 | 3.5 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 443.0 | 31.0 | 7.0 |
| 2 | 16 | 114.0 | 6.3 | 5.5 |
| 3 | 16 | 60.4 | 3.1 | 5.1 |
Background Information
ANGPTL3 is a secreted glycoprotein primarily synthesized by hepatocytes and belongs to the angiopoietin-like protein family. ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis, and hematopoietic function, and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. ANGPTL3 inhibits low-density lipoprotein (LDL) and endothelial lipase (EL), two enzymes that hydrolyze triglycerides and phospholipids in plasma lipoproteins. ANGPTL3-deficient mice showed low plasma HDL cholesterol and HDL phospholipid (PL). ANGPTL3 can also regulate triglyceride metabolism by inhibiting lipoprotein lipase (LPL). ANGPTL3-deficient mice exhibit hypotriglyceridemia and elevated LPL activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Angptl3, angiopoietin like 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.