Mouse Atox1 ELISA Kit
Cat no : KE10153
Synonyms
Atox1, Atx1, HAH1
Validation Data Gallery
Product Information
KE10153 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Atox1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Atox1. The assay recognizes mouse Atox1. An antibody specific for mouse Atox1 has been pre-coated onto the microwells. The mouse Atox1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Atox1 is added to detect the captured mouse Atox1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Atox1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11927 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 84% | 82%-87% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,850.0 | 97.3 | 3.4 |
| 2 | 8 | 756.3 | 21.2 | 2.8 |
| 3 | 8 | 214.2 | 6.2 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,983.0 | 178.8 | 6.0 |
| 2 | 16 | 782.6 | 37.2 | 4.8 |
| 3 | 16 | 214.4 | 16.3 | 7.6 |
Background Information
Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Atox1, Atx1, HAH1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.