Mouse B7-H2/ICOSLG ELISA Kit
Cat no : KE10114
Synonyms
AU044799, B7 H2, B7h, B7RP 1, BG071784, GI50, GL50, GL50 B, ICOS L, icos ligand, Icosl, Icoslg, KIAA0653, LICOS, Ly115l, mKIAA0653
Validation Data Gallery
Product Information
KE10114 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse B7-H2/ICOSLG ELISA kit is to be used to detect and quantify protein levels of endogenous mouse B7-H2/ICOSLG. The assay recognizes mouse B7-H2/ICOSLG. An antibody specific for mouse B7-H2/ICOSLG has been pre-coated onto the microwells. The mouse B7-H2/ICOSLG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse B7-H2/ICOSLG is added to detect the captured mouse B7-H2/ICOSLG protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse B7-H2/ICOSLG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 5.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 50723 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 96% | 88%-106% |
| Cell culture supernatants | 108% | 96%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,272.6 | 115.0 | 5.1 |
| 2 | 20 | 494.7 | 16.2 | 3.3 |
| 3 | 20 | 111.3 | 3.9 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,097.9 | 101.2 | 4.8 |
| 2 | 24 | 487.7 | 17.0 | 3.5 |
| 3 | 24 | 115.8 | 5.7 | 5.0 |
Background Information
B7-H2, also known as ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. B7-H2/ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells. It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule. The interaction of B7-H2/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AU044799, B7 H2, B7h, B7RP 1, BG071784, GI50, GL50, GL50 B, ICOS L, icos ligand, Icosl, Icoslg, KIAA0653, LICOS, Ly115l, mKIAA0653 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.