Mouse CCL12/MCP-5 ELISA Kit
Cat no : KE10051
Synonyms
Ccl12, MCP 5, MCP-5, Scya12
Validation Data Gallery
Product Information
KE10051 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL12/MCP-5 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL12/MCP-5. The assay recognizes mouse CCL12/MCP-5. An antibody specific for CCL12/MCP-5 has been pre-coated onto the microwells. The CCL12/MCP-5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL12/MCP-5 is added to detect the captured mouse CCL12/MCP-5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CCL12/MCP-5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.06 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 90% | 80%-97% |
| Cell culture supernatants | 97% | 85%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 116.3 | 2.9 | 2.5 |
| 2 | 20 | 26.8 | 0.9 | 3.4 |
| 3 | 20 | 5.5 | 0.1 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 115.0 | 2.3 | 2.0 |
| 2 | 24 | 26.1 | 0.6 | 2.4 |
| 3 | 24 | 6.2 | 0.2 | 3.3 |
Background Information
CCL12, also known as MCP-5, is a small cytokine belonging to the CC chemokine family. It is a structural and functional homologue of human MCP-1. CCL12 is found predominantly in lymph nodes and thymus under normal conditions, and its expression can be markedly induced in activated macrophages both in vitro and in vivo. CCL12 is involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl12, MCP 5, MCP-5, Scya12 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.