Mouse CCL17/TARC ELISA Kit
Cat no : KE10096
Synonyms
Abcd 2, Ccl17, Scya17, Scya17l, Tarc
Validation Data Gallery
Product Information
KE10096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CCL17/TARC ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL17/TARC. The assay recognizes mouse CCL17/TARC. An antibody specific for mouse CCL17/TARC has been pre-coated onto the microwells. The mouse CCL17/TARC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL17/TARC is added to detect the captured mouse CCL17/TARC protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CCL17/TARC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20295 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 91% | 71%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 777.4 | 69.6 | 9.0 |
| 2 | 20 | 213.8 | 7.0 | 3.3 |
| 3 | 20 | 51.9 | 1.7 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 714.1 | 71.6 | 10.0 |
| 2 | 24 | 220.4 | 9.8 | 4.4 |
| 3 | 24 | 50.4 | 2.1 | 4.3 |
Background Information
Thymus and Activation-Regulated Chemokine (TARC/CCL17) is a member of the CC or β-chemokine family. TARC/CCL17 is constitutively expressed in the thymus and is produced by dendritic cells (DC), endothelial cells, keratinocytes (KC) and fibroblasts. It is a ligand for CCR4, which is predominantly expressed on Th2 lymphocytes, basophils and natural killer cells. TARC has been shown to selectively recruit skin-homing CLA+ memory CD4+ T cells into skin during times of immune challenge. The serum TARC levels are closely related to the disease activity of Atopic dermatitis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Abcd 2, Ccl17, Scya17, Scya17l, Tarc |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Med Immune Cell Profiling Reveals a Common Pattern in Premetastatic Niche Formation Across Various Cancer Types |