Mouse CCL5 ELISA Kit

Sensitivity

5.7 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10017

Synonyms

C-C motif chemokine 5, Ccl5, chemokine (C C motif) ligand 5, MuRantes, RANTES, Scya5, SISd, Small-inducible cytokine A5, TCP228



Product Information

KE10017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL5. The assay recognizes mouse CCL5. An antibody specific for mouse CCL5 has been pre-coated onto the microwells. The mouse CCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL5 is added to detect the captured mouse CCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse CCL5 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 5.7 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum87%74%-124%
Cell culture supernatants106%83%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12041.42.45.8
220162.05.23.2
320523.049.29.3

InterAssay

Samplenmean ( pg/mL)SDCV%
12455.85.39.5
224259.123.89.2
324905.658.16.4

Background Information

CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms C-C motif chemokine 5, Ccl5, chemokine (C C motif) ligand 5, MuRantes, RANTES, Scya5, SISd, Small-inducible cytokine A5, TCP228

Publications

SpeciesSample TypeTitle
mouseserum

FEBS J

Deficiency of p38α in macrophage ameliorates d-galactosamine/TNF-α-induced acute liver injury in mice.

Authors - Jiao Liu