Mouse CD13 ELISA Kit
Cat no : KE10203
Synonyms
Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Anpep, AP-M
Validation Data Gallery
Product Information
KE10203 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD13 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD13. The assay recognizes mouse CD13. An antibody specific for mouse CD13 has been pre-coated onto the microwells. The mouse CD13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD13 is added to detect the captured mouse CD13 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16790 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 91% | 86%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 12.26 | 0.60 | 4.89 |
| 2 | 8 | 3.01 | 0.09 | 3.09 |
| 3 | 8 | 1.60 | 0.05 | 2.84 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.14 | 1.09 | 9.01 |
| 2 | 16 | 2.96 | 0.11 | 3.67 |
| 3 | 16 | 1.60 | 0.06 | 3.55 |
Background Information
CD13 is a type II transmembrane glycoprotein that is involved in the processing of various peptides, including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. CD13 may also be involved in the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen-presenting cells. It could be a potential therapeutic target for inflammatory disorders. Additionally, soluble CD13 (sCD13) can bind to a G-protein-coupled receptor on activated T cells, monocytes, endothelial cells, and fibroblasts, acting as a chemoattractant for monocyte and T cell migration.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Anpep, AP-M |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.