Mouse CD25/IL-2RA ELISA Kit
Cat no : KE10208
Synonyms
CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra
Validation Data Gallery
Product Information
KE10208 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD25/IL-2RA ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD25/IL-2RA. The assay recognizes mouse CD25/IL-2RA. An antibody specific for mouse CD25/IL-2RA has been pre-coated onto the microwells. The mouse CD25/IL-2RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD25/IL-2RA is added to detect the captured mouse CD25/IL-2RA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD25/IL-2RA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16184 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 77% | 73%-83% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,448.1 | 67.6 | 4.7 |
| 2 | 8 | 394.0 | 11.2 | 2.8 |
| 3 | 8 | 171.6 | 6.8 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,461.8 | 55.5 | 3.8 |
| 2 | 16 | 381.4 | 17.2 | 4.5 |
| 3 | 16 | 175.6 | 7.7 | 4.4 |
Background Information
CD25, also known as the α chain of the IL-2 receptor (IL-2Rα), is a key regulator of T cell function. It is expressed in activated T cells and has been implicated in regulating various immune and autoimmune responses. Its deficiency has been associated with autoimmune phenomena including autoimmune enteropathy and bullous pemphigoid. The soluble CD25 can be found in the serum and may have implications for human disease in both cancer and chronic infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.