Mouse CD34 ELISA Kit
Cat no : KE10138
Synonyms
Cd34,CD34 antigen
Validation Data Gallery
Product Information
KE10138 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD34 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD34. The assay recognizes mouse CD34. An antibody specific for mouse CD34 has been pre-coated onto the microwells. The mouse CD34 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD34 is added to detect the captured mouse CD34 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD34 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12490 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 99%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 532.0 | 32.8 | 6.2 |
| 2 | 20 | 128.0 | 5.8 | 4.5 |
| 3 | 20 | 31.5 | 1.4 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 504.0 | 31.8 | 6.3 |
| 2 | 24 | 117.4 | 6.0 | 5.1 |
| 3 | 24 | 28.9 | 1.1 | 3.9 |
Background Information
CD34 is a 105- to 120-kDa transmembrane phosphoglycoprotein consisting of a cytoplasmic tail that contains PDZ-domain binding motifs, a single transmembrane helix and an extracellular domain that is heavily sialylated, O-linked glycosylated, and contains some N-linked glycosylation sites. It is expressed by the majority of hematopoietic stem/progenitor cells and also by a multitude of other nonhematopoietic cell types including muscle satellite cells, corneal keratocytes, interstitial cells, epithelial progenitors, and vascular endothelial cells. CD34 is widely used as a marker of vascular endothelial cells and hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd34,CD34 antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.