CD45.1 Recombinant antibody

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  WB analysis using 84325-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84325-1-RR (Mouse CD45.1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 84325-1-RR

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse spleen tissue using CD45.1 antibody (84325-1-RR, Clone: 240921B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD45.1 Uni-rAb^TM Recombinant Antibody for WB, IF-Fro, ELISA

Cat No. 84325-1-RR

Clone No.240921B5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-Fro, ELISA

Synonyms

Ly5a, Cd45, Ptprc, 240921B5, B220

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Tested Applications

• Positive WB detected in: SJL mouse thymus tissue, SJL mouse spleen tissue
• Positive IF-Fro detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84325-1-RR targets CD45.1 in WB, IF-Fro, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor type, C
• Observed Molecular Weight: 220 kDa
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).