Mouse CD45 ELISA Kit
Cat no : KE10140
Synonyms
Cd45,Ptprc,B220,LCA,Leukocyte common antigen
Validation Data Gallery
Product Information
KE10140 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD45 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD45. The assay recognizes mouse CD45. An antibody specific for mouse CD45 has been pre-coated onto the microwells. The mouse CD45 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD45 is added to detect the captured mouse CD45 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD45 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19264 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 97% | 96%-99% |
| Cell lysate | 100% | 98%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 250.9 | 5.6 | 2.2 |
| 2 | 20 | 59.7 | 1.3 | 2.1 |
| 3 | 20 | 15.1 | 0.6 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 235.6 | 11.9 | 5.1 |
| 2 | 24 | 57.2 | 2.5 | 4.4 |
| 3 | 24 | 15.3 | 1.0 | 6.8 |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets. CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd45,Ptprc,B220,LCA,Leukocyte common antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.