Mouse CD5L ELISA Kit
Cat no : KE10218
Synonyms
Aim, Api6, Apoptosis inhibitor expressed by macrophages, Apoptosis inhibitory 6, CD5 antigen-like
Validation Data Gallery
Product Information
KE10218 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD5L ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD5L. The assay recognizes mouse CD5L. An antibody specific for mouse CD5L has been pre-coated onto the microwells. The mouse CD5L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD5L is added to detect the captured mouse CD5L protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD5L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11801 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 116% | 109%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.27 | 0.59 | 6.42 |
| 2 | 8 | 2.58 | 0.13 | 5.08 |
| 3 | 8 | 1.36 | 0.08 | 5.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.81 | 0.92 | 9.36 |
| 2 | 16 | 2.59 | 0.14 | 5.45 |
| 3 | 16 | 1.30 | 0.11 | 8.20 |
Background Information
CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins. CD5L is a soluble protein mainly secreted by macrophages in lymphoid and inflamed tissues. It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. Additionally, CD5L is involved in different processes such as metabolism, infection, and autophagy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Aim, Api6, Apoptosis inhibitor expressed by macrophages, Apoptosis inhibitory 6, CD5 antigen-like |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.