Mouse CD73 ELISA Kit
Cat no : KE10174
Synonyms
5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase
Validation Data Gallery
Product Information
KE10174 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD73 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD73. The assay recognizes mouse CD73. An antibody specific for mouse CD73 has been pre-coated onto the microwells. The mouse CD73 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD73 is added to detect the captured mouse CD73 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD73 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23959 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 100% | 97%-101% |
| Tissue homogenates | 100% | 97%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,155.8 | 56.3 | 2.6 |
| 2 | 8 | 561.0 | 25.0 | 4.5 |
| 3 | 8 | 170.9 | 5.9 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,269.5 | 128.0 | 5.6 |
| 2 | 16 | 561.0 | 20.1 | 3.6 |
| 3 | 16 | 170.9 | 5.7 | 3.3 |
Background Information
CD73 is a membrane-bound enzyme that catalyzes the extracellular hydrolysis of adenosine monophosphate (AMP) to produce adenosine. It is expressed on various immune cells, including T cells, B cells, dendritic cells, and regulatory T cells (Tregs). CD73 works in conjunction with CD39 to break down extracellular ATP into adenosine, creating an immunosuppressive environment that can inhibit the function of cytotoxic T cells and natural killer (NK) cells. This process is essential for immune tolerance and the resolution of inflammation. CD73 is also an important player in controlling tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase |