Mouse CD79B ELISA Kit
Cat no : KE10152
Synonyms
B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, CD79b, Igb, Ig-beta
Validation Data Gallery
Product Information
KE10152 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD79B ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD79B. The assay recognizes mouse CD79B. An antibody specific for mouse CD79B has been pre-coated onto the microwells. The mouse CD79B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD79B is added to detect the captured mouse CD79B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD79B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15985 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue homogenate | 97% | 89%-105% |
| Tissue lysate | 95% | 90%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,759.4 | 78.0 | 2.8 |
| 2 | 8 | 824.6 | 19.2 | 2.3 |
| 3 | 8 | 232.2 | 21.3 | 9.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,681.4 | 131.2 | 4.9 |
| 2 | 16 | 769.8 | 61.5 | 8.0 |
| 3 | 16 | 230.1 | 20.8 | 9.0 |
Background Information
CD79 is composed of CD79a and CD79b components expressed almost exclusively on B cells and B-cell neoplasms. The transmembrane CD79a and CD79b proteins couple at the extracellular end with any one of the five different types of transmembrane Ig molecules (IgM, IgD, IgG, IgE, or IgA), which are disulfide-linked proteins composed of two Ig heavy chains and two Ig light chains. The intracytoplasmic ends of the CD79a/CD79b proteins can couple with intracellular signaling components as well. Furthermore, Activated B lymphocytes have increased amounts of the truncated CD79b. Loss of CD79b expression is a characteristic feature of CLL/SLL and may be of diagnostic use.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, CD79b, Igb, Ig-beta |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.