Mouse CD80/B7-1 ELISA Kit
Cat no : KE10180
Synonyms
Cd80, B7 1, B7.1, Cd28l, CD80 antigen
Validation Data Gallery
Product Information
KE10180 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD80/B7-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD80/B7-1. The assay recognizes mouse CD80/B7-1. An antibody specific for mouse CD80/B7-1 has been pre-coated onto the microwells. The mouse CD80/B7-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD80/B7-1 is added to detect the captured mouse CD80/B7-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD80/B7-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12519 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 97%-110% |
| Cell lysate | 112% | 99%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 184.8 | 5.9 | 3.2 |
| 2 | 8 | 49.6 | 3.7 | 7.4 |
| 3 | 8 | 29.4 | 2.4 | 8.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 188.0 | 10.0 | 5.3 |
| 2 | 16 | 51.1 | 4.3 | 8.4 |
| 3 | 16 | 28.6 | 2.6 | 9.1 |
Background Information
CD80, also known as B7-1, is a type I membrane protein and a member of the immunoglobulin superfamily. It plays a crucial role in T cell activation and regulation by providing a co-stimulatory signal necessary for T cell proliferation and cytokine production. CD80 interacts with CD28 on T cells to promote T cell activation and with CTLA-4 to inhibit T cell activation. This dual role highlights its importance in modulating immune responses. Additionally, CD80 acts as a receptor for adenovirus subgroup B, indicating its involvement in viral infections.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd80, B7 1, B7.1, Cd28l, CD80 antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.