Mouse CEACAM1/CD66a ELISA Kit
Cat no : KE10178
Synonyms
Bgp, Bgp1, BGP-1, Biliary glycoprotein 1, Biliary glycoprotein D
Validation Data Gallery
Product Information
KE10178 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CEACAM1/CD66a ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CEACAM1/CD66a. The assay recognizes mouse CEACAM1/CD66a. An antibody specific for mouse CEACAM1/CD66a has been pre-coated onto the microwells. The mouse CEACAM1/CD66a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CEACAM1/CD66a is added to detect the captured mouse CEACAM1/CD66a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CEACAM1/CD66a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 5.2 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 26365 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 98% | 91%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,222.4 | 39.2 | 3.2 |
| 2 | 8 | 300.6 | 7.2 | 2.4 |
| 3 | 8 | 152.5 | 6.7 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,223.3 | 36.1 | 2.9 |
| 2 | 16 | 298.7 | 7.6 | 2.5 |
| 3 | 16 | 152.7 | 7.4 | 4.9 |
Background Information
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, also known as CD66a) is a member of the immunoglobulin superfamily and belongs to the carcinoembryonic antigen (CEA) family. It is a highly glycosylated cell surface glycoprotein that mediates cell adhesion via homophilic and heterophilic interactions. CEACAM1 plays crucial roles in various cellular processes, including tissue differentiation, angiogenesis, apoptosis, tumor suppression, and the modulation of both innate and adaptive immune responses. It is also involved in negative regulation of the T cell receptor signaling pathway, negative regulation of leukocyte-mediated cytotoxicity, and wound healing.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bgp, Bgp1, BGP-1, Biliary glycoprotein 1, Biliary glycoprotein D |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.