Mouse CHI3L1/YKL40 ELISA Kit
Cat no : KE10090
Synonyms
AW208766, Breast regression protein 39, Brp39, Cartilage glycoprotein 39, CGP 39, Chi3l1, Chil1, chitinase 3 like 1, GP 39, Gp39, YKL40
Validation Data Gallery
Product Information
KE10090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CHI3L1/YKL40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CHI3L1/YKL40. The assay recognizes mouse CHI3L1/YKL40. An antibody specific for mouse CHI3L1/YKL40 has been pre-coated onto the microwells. The mouse CHI3L1/YKL40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CHI3L1/YKL40 is added to detect the captured mouse CHI3L1/YKL40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CHI3L1/YKL40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12654 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 104% | 84%-124% |
| Cell culture supernatants | 89% | 74%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 185.3 | 6.6 | 3.6 |
| 2 | 20 | 46.5 | 1.8 | 3.9 |
| 3 | 20 | 9.6 | 0.7 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 187.2 | 8.0 | 4.3 |
| 2 | 24 | 45.6 | 1.5 | 3.4 |
| 3 | 24 | 10.7 | 1.0 | 9.6 |
Background Information
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39) is a glycoprotein synthesized and secreted by a multitude of cells including macrophages, neutrophils, synoviocytes, chondrocytes, fibroblast-like cells, smooth muscle cells, and tumor cells. It plays a major role in tissue injury, inflammation, tissue repair, and remodeling responses. CHI3L1 has been strongly associated with diseases including asthma, arthritis, sepsis, diabetes, liver fibrosis, and coronary artery disease. CHI3L1 signaling plays a critical role in cancer cell growth, proliferation, invasion, metastasis, angiogenesis, activation of tumor-associated macrophages, and Th2 polarization of CD4+ T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AW208766, Breast regression protein 39, Brp39, Cartilage glycoprotein 39, CGP 39, Chi3l1, Chil1, chitinase 3 like 1, GP 39, Gp39, YKL40 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.