Mouse CRTAM/CD355 ELISA Kit
Cat no : KE10221
Synonyms
CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule
Validation Data Gallery
Product Information
KE10221 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CRTAM/CD355 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CRTAM/CD355. The assay recognizes mouse CRTAM/CD355. An antibody specific for mouse CRTAM/CD355 has been pre-coated onto the microwells. The mouse CRTAM/CD355 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CRTAM/CD355 is added to detect the captured mouse CRTAM/CD355 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CRTAM/CD355 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54698 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 83% | 82%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.88 | 0.25 | 5.03 |
| 2 | 8 | 1.22 | 0.05 | 3.94 |
| 3 | 8 | 0.38 | 0.02 | 4.01 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.78 | 0.29 | 6.09 |
| 2 | 16 | 1.22 | 0.04 | 3.65 |
| 3 | 16 | 0.38 | 0.02 | 4.08 |
Background Information
Cytotoxic and regulatory T-cell molecule (CRTAM, also known as CD355) has been characterized as an important activation marker for both CD8+ T cells and NK-T cells. Engagement of CRTAM promotes cytotoxic effects of NK cells towards Necl-2 expressing tumor cells. Coligation of TCR and CRTAM on CD8+ T cells causes interferon-c (IFN-c) secretion in vitro. Aside from the presence in cells of the immune system and its immunological functions, CRTAM is also involved in cell-cell adhesion due to its expression in epithelial cells and has a role in the transcytosis of neural stem cells across brain vascular endothelial cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD355, Class-I MHC-restricted T-cell-associated molecule, Crtam, Cytotoxic and regulatory T-cell molecule |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.