Mouse CXCL13/BCA1 ELISA Kit
Cat no : KE10088
Synonyms
Cxcl13,B lymphocyte chemoattractant,BCA1,Blc,CXC c
Validation Data Gallery
Product Information
KE10088 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CXCL13/BCA1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CXCL13/BCA1. The assay recognizes mouse CXCL13/BCA1. An antibody specific for mouse CXCL13/BCA1 has been pre-coated onto the microwells. The mouse CXCL13/BCA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CXCL13/BCA1 is added to detect the captured mouse CXCL13/BCA1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CXCL13/BCA1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55985 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 82% | 69%-108% |
| Cell culture supernatants | 108% | 99%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 540.7 | 19.1 | 3.5 |
| 2 | 20 | 130.7 | 4.3 | 3.3 |
| 3 | 20 | 26.7 | 1.4 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 502.6 | 19.6 | 3.9 |
| 2 | 24 | 124.8 | 6.3 | 5.1 |
| 3 | 24 | 27.0 | 1.8 | 6.6 |
Background Information
Chemokine (C-X-C motif) ligand 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC) or B cell-attracting chemokine 1 (BCA1), is a protein ligand. CXCL13 is chemotactic for B cells belonging to both the B-1 and B-2 subsets, and effects by interacting with chemokine receptor CXCR5. CCL19, CCL21 and CXCL13 expression are related to local immune responses to influenza virus infection in mice lung. CXCL13 also acts as inducers in early innate immunity and formation of protective lymphoid follicles within granulomas. CXCL13 expression has correlation with the pathogenesis of aberrant lymphoid structures, and acts as unfavorable prognosis in multiple sclerosis, Sjögren's, myasthenia gravis, and SLE.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cxcl13,B lymphocyte chemoattractant,BCA1,Blc,CXC c |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Med LILRB1 Is a Prognostic-Related Biomarker Correlated With Immune Infiltration in Head-Neck Squamous Cell Carcinoma. |