Mouse CXCL2 ELISA Kit
Cat no : KE10022
Synonyms
C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2, Scyb2
Validation Data Gallery
Product Information
KE10022 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL2. The assay recognizes mouse CXCL2. An antibody specific for CXCL2 has been pre-coated onto the microwells. The CXCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL2 is added to detect the captured CXCL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CXCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 20.5 pg/mL |
| Range | 31.25-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20310 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 86% | 72%-133% |
| Cell culture supernatants | 100% | 82%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 438.8 | 13.6 | 3.1 |
| 2 | 20 | 137.6 | 4.4 | 3.2 |
| 3 | 20 | 30.7 | 1.6 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 443.1 | 12.1 | 2.7 |
| 2 | 24 | 145.2 | 4.4 | 3.0 |
| 3 | 24 | 30.0 | 1.5 | 5.1 |
Background Information
Cxcl2, also known as macrophage inflammatory protein 2-alpha (Mip2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (Cxcr2). Cxcl2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. Cxcl2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete Cxcl2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that Cxcl2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2, Scyb2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades. | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Cancer Lett CircRREB1 promotes gastrointestinal cancer metastasis and MDSC-controlled immune evasion. | ||
Oncogene CUL4B functions as a tumor suppressor in KRAS-driven lung tumors by inhibiting the recruitment of myeloid-derived suppressor cells | ||
Stem Cell Res Ther Enhanced therapeutic efficacy of repeated bone marrow-derived MSC administration in a murine model of pulmonary fibrosis. | ||
Chin Med Thlaspi arvense attenuates colitis-associated colorectal tumorigenesis through suppression of neutrophil recruitment via the NOD/NF-κB pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Dan (Verified Customer) (02-27-2026) | CXCL2 ELISA done on mouse serum from animals receiving either control or gene therapy X via intramuscular injection
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