Mouse DPP4/CD26 ELISA Kit
Cat no : KE10200
Synonyms
CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV
Validation Data Gallery
Product Information
KE10200 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse DPP4/CD26 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse DPP4/CD26. The assay recognizes mouse DPP4/CD26. An antibody specific for mouse DPP4/CD26 has been pre-coated onto the microwells. The mouse DPP4/CD26 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse DPP4/CD26 is added to detect the captured mouse DPP4/CD26 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse DPP4/CD26 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13482 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 77% | 74%-80% |
| Mouse urine | 88% | 76%-101% |
| Cell culture supernatant | 78% | 76%-80% |
| Tissue lysate | 90% | 85%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 13.45 | 0.43 | 3.19 |
| 2 | 8 | 3.13 | 0.14 | 4.58 |
| 3 | 8 | 0.99 | 0.04 | 3.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 13.43 | 0.60 | 4.49 |
| 2 | 16 | 3.21 | 0.16 | 5.04 |
| 3 | 16 | 1.00 | 0.06 | 5.80 |
Background Information
Dipeptidyl peptidase 4 (DPP4, also known as CD26) is a type II transmembrane glycoprotein with enzymatic activity. DPP4 plays a crucial role in immune regulation, T-cell activation, and the regulation of chemokine function. DPP4 also interacts with adenosine deaminase (ADA) and the chemokine receptor CXCR4, influencing immune responses and cell migration. Additionally, circulating soluble DPP4 (sDPP4) is released from adipose tissue and acts as a pro-inflammatory adipokine, linking the expansion of adipose tissue with increased inflammation, insulin resistance, and metabolic syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.