Mouse DR5 ELISA Kit
Cat no : KE10154
Synonyms
CD262, Death receptor 5, Killer, MK, Tnfrsf10b
Validation Data Gallery
Product Information
KE10154 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse DR5 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse DR5. The assay recognizes mouse DR5. An antibody specific for mouse DR5 has been pre-coated onto the microwells. The mouse DR5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse DR5 is added to detect the captured mouse DR5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse DR5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21933 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 99% | 94%-104% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.86 | 0.58 | 5.85 |
| 2 | 8 | 2.59 | 0.10 | 3.98 |
| 3 | 8 | 1.53 | 0.06 | 3.91 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.14 | 0.82 | 8.09 |
| 2 | 16 | 2.64 | 0.14 | 5.44 |
| 3 | 16 | 1.57 | 0.08 | 5.32 |
Background Information
DR5 (Death Receptor 5) is a member of the tumor necrosis factor receptor superfamily, which activates apoptosis signaling pathway mainly through binding to TNF-related apoptosis-inducing ligand (TRAIL). DR5 is expressed in a variety of cell types, including cancer cells and immune cells, and its activation induces apoptosis, thus it is considered as a potential anti-tumor target. In recent years, it has been found that DR5 is not only involved in apoptosis, but also plays an important role in antiviral immunity. For example, DR5 is able to inhibit the replication of human herpesvirus by inducing apoptosis. In addition, activation of DR5 is involved in the integrative stress response (ISR), where the expression of DR5 is increased and ligand-independent activation of the apoptotic pathway under cellular stress conditions. These studies provide new ideas for the development of DR5-based antitumor and antiviral therapies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD262, Death receptor 5, Killer, MK, Tnfrsf10b |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.