Mouse EPCAM/CD326 ELISA Kit
Cat no : KE10179
Synonyms
CD326, EGP314, Epcam, Ep-CAM, Epithelial cell adhesion molecule
Validation Data Gallery
Product Information
KE10179 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse EPCAM/CD326 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse EPCAM/CD326. The assay recognizes mouse EPCAM/CD326. An antibody specific for mouse EPCAM/CD326 has been pre-coated onto the microwells. The mouse EPCAM/CD326 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse EPCAM/CD326 is added to detect the captured mouse EPCAM/CD326 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse EPCAM/CD326 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 4.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17075 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 103% | 101%-105% |
| Cell lysate | 91% | 82%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 754.9 | 22.4 | 3.0 |
| 2 | 8 | 186.9 | 4.4 | 2.4 |
| 3 | 8 | 84.8 | 3.8 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 760.2 | 19.9 | 2.6 |
| 2 | 16 | 186.8 | 5.6 | 3.0 |
| 3 | 16 | 88.7 | 5.3 | 6.0 |
Background Information
Epithelial cell adhesion molecule (EPCAM), also known as CD326, is a well-characterized type I transmembrane glycoprotein that plays a pivotal role in cell adhesion, proliferation, differentiation, and signaling. It mediates Ca2+-independent homotypic cell-cell adhesion through its extracellular domain, which is crucial for the formation and maintenance of epithelial tissues. The membrane localization of EPCAM is essential for its function in cell adhesion and signaling. Additionally, elevated levels of soluble EPCAM have been associated with tumor burden and poor prognosis in cancer patients, making it a potential biomarker for cancer diagnosis and monitoring.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD326, EGP314, Epcam, Ep-CAM, Epithelial cell adhesion molecule |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.